beta II Tubulin A Antibody (3C12)

Images

 
Western Blot: beta II Tubulin A Antibody (3C12) [H00007280-M02] - TUBB2A monoclonal antibody (M02), clone 3C12. Analysis of TUBB2A expression in Jurkat.
Immunohistochemistry-Paraffin: beta II Tubulin A Antibody (3C12) [H00007280-M02] - Analysis of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. Antibody concentration 6 ug/ml.
Western Blot: beta II Tubulin A Antibody (3C12) [H00007280-M02] - TUBB2A monoclonal antibody (M02), clone 3C12. Analysis of TUBB2A expression in PC-12.
Western Blot: beta II Tubulin A Antibody (3C12) [H00007280-M02] - TUBB2A monoclonal antibody (M02), clone 3C12. Analysis of TUBB2A expression in NIH/3T3.
Sandwich ELISA: beta II Tubulin A Antibody (3C12) [H00007280-M02] - Detection limit for recombinant GST tagged TUBB2A is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC-P
Clone
3C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta II Tubulin A Antibody (3C12) Summary

Immunogen
TUBB2A (AAH01194, 1 a.a. - 445 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNTNDLVSEYQQYQDATADEQGEFEEEEGEDEA
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBB2A
Purity
IgG purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
Application Notes
It has been used for ELISA and WB.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta II Tubulin A Antibody (3C12)

  • dJ40E16.7
  • TUBB
  • TUBB2
  • TUBB2B
  • tubulin beta-2A chain
  • tubulin, beta 2
  • tubulin, beta 2A
  • tubulin, beta polypeptide 2
  • tubulin, beta polypeptide

Background

Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TUBB2A
Uniprot