beta II Tubulin A Antibody (3B2) - Azide and BSA Free

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in Jurkat (Cat # L017V1).

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of monoclonal antibody to TUBB2A on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in PC-12 (Cat # L012V1).

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in Raw 264.7 (Cat # L024V1).

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in NIH/3T3 (Cat # L018V1).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

beta II Tubulin A Antibody (3B2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TUBB2A (AAH01194, 1 a.a. ~ 445 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNTNDLVSEYQQYQDATADEQGEFEEEEGEDEA
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB2A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: It has been used for ELISA, WB and IF.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta II Tubulin A Antibody (3B2) - Azide and BSA Free

• beta II Tubulin A
• dJ40E16.7
• TUBB
• TUBB2
• TUBB2A
• TUBB2B
• tubulin beta-2A chain
• tubulin, beta 2
• tubulin, beta 2A
• tubulin, beta polypeptide 2
• tubulin, beta polypeptide

Background

Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB2A
• Uniprot:
  • AAH01194(Human)