Mouse beta I Tubulin ELISA Kit (Colorimetric) Summary
| Description |
Assay time: 3 hours |
| Standard Curve Range |
0.156 - 10 ng/mL (example only; lot dependent) |
| Sensitivity |
0.063 ng/mL (example only; lot dependent) |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
%CV < 12 (example only; lot dependent) |
| Intra-Assay |
%CV < 10 (example only; lot dependent) |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TUBB1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard Diluent
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Mouse beta I Tubulin ELISA Kit (Colorimetric)
Background
Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for beta I Tubulin ELISA Kit (NBP3-42617). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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