beta I + II Tubulin Antibody (JDR.3B8)

Product Discontinued

beta I + II Tubulin Antibody (JDR.3B8) Summary

• Immunogen: A chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype II coupled to BSA.
• Epitope: Ab11313 specifically recognizes an epitope located on the isotypes I and II of human beta-tubulin.
• Localization: Tubulin is the major constituent of microtubules.
• Specificity: Using the immunoblotting technique, the localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line. The antibody specifically recognizes an epitope located on isotypes I and II of beta -tubulin. No cross-reactivity with other tubulin isotypes is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: IF: Use at an assay dependent dilution. The antibody localizes the beta-tubulin isotypes I and II in human and chicken fibroblasts.WB: Use at a dilution of 1/1000. The antibody localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line.Predicted molecular weight: 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Cow, Chicken and Pig.Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Raw ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for beta I + II Tubulin Antibody (JDR.3B8)

• beta tubulin 1, class VI
• dJ543J19.4
• tubulin beta-1 chain
• tubulin, beta 1

Background

Tubulin is the major building block of microtubules. This intracellular, cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar but not identical sets of alpha and beta tubulin polypeptides. Within either family, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxy-terminal 15 residues. For beta-tubulin, five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species with the exception of the hematopoietic beta tubulin which is highly divergent in sequence and which is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB1
• Entrez:
  • 498736(Rat)