beta-Glucuronidase/GUSB Overexpression Lysate (Native)

Images

 
Western Blot: beta Glucuronidase Overexpression Lysate (Adult Normal) [NBL1-11412] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for beta Glucuronidase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

beta-Glucuronidase/GUSB Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204325 and based on accession number NM_000181. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens glucuronidase, beta (GUSB), mRNA.
Gene
GUSB

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
72.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for beta-Glucuronidase/GUSB

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for beta-Glucuronidase/GUSB Overexpression Lysate (Native)

  • beta-D-glucuronidase
  • Beta-G1
  • beta-glucuronidase
  • BG
  • EC 3.2.1.31
  • FLJ39445
  • glucuronidase, beta
  • GUSB
  • MPS7

Background

The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for beta-Glucuronidase/GUSB Lysate (NBL1-11412) (0)

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Bioinformatics

Gene Symbol GUSB
Entrez