beta-Glucuronidase/GUSB Overexpression Lysate

Images

 
Western Blot: beta-Glucuronidase/GUSB Overexpression Lysate [NBL1-11412] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for beta Glucuronidase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

beta-Glucuronidase/GUSB Overexpression Lysate Summary

Description

beta-Glucuronidase/GUSB Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204325

Accession#: NM_000181

Protein Tag: C-MYC/DDK
Gene
GUSB

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
72.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for beta-Glucuronidase/GUSB Overexpression Lysate

  • beta-D-glucuronidase
  • Beta-G1
  • beta-glucuronidase
  • BG
  • EC 3.2.1.31
  • FLJ39445
  • glucuronidase, beta
  • GUSB
  • MPS7

Background

The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-45731
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-61118
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
H00347527-P01
Species: Hu
Applications: ELISA, AP, PA, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
AF3667
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
AF1029
Species: Mu
Applications: ICC, IHC, IP, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
664-LI/CF
Species: Hu
Applications: BA
NBP1-86615
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NB100-56473
Species: Hu, Mu, Pm
Applications: Simple Western, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-31555
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
D6050
Species: Hu
Applications: ELISA
NBL1-11412
Species: Hu
Applications: WB

Publications for beta-Glucuronidase/GUSB Lysate (NBL1-11412) (0)

There are no publications for beta-Glucuronidase/GUSB Lysate (NBL1-11412).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta-Glucuronidase/GUSB Lysate (NBL1-11412) (0)

There are no reviews for beta-Glucuronidase/GUSB Lysate (NBL1-11412). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Glucuronidase/GUSB Lysate (NBL1-11412) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional beta-Glucuronidase/GUSB Products

Bioinformatics Tool for beta-Glucuronidase/GUSB Lysate (NBL1-11412)

Discover related pathways, diseases and genes to beta-Glucuronidase/GUSB Lysate (NBL1-11412). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta-Glucuronidase/GUSB Lysate (NBL1-11412)

Discover more about diseases related to beta-Glucuronidase/GUSB Lysate (NBL1-11412).
 

Pathways for beta-Glucuronidase/GUSB Lysate (NBL1-11412)

View related products by pathway.

PTMs for beta-Glucuronidase/GUSB Lysate (NBL1-11412)

Learn more about PTMs related to beta-Glucuronidase/GUSB Lysate (NBL1-11412).
 

Research Areas for beta-Glucuronidase/GUSB Lysate (NBL1-11412)

Find related products by research area.

Blogs on beta-Glucuronidase/GUSB

There are no specific blogs for beta-Glucuronidase/GUSB, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our beta-Glucuronidase/GUSB Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GUSB