beta-3 Adrenergic R/ADRB3 Antibody

Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [NB100-93369] - (0.03ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

beta-3 Adrenergic R/ADRB3 Antibody Summary

• Immunogen: Peptide with sequence PHENSSLAPWPD-C corresponding to N-Terminus according to NP_000016.1.
• Epitope: PHENSSLAPWPD-C
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADRB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in human brain (cerebellum, cerebral cortex, frontal cortex) lysates (calculated MW of 43.5 kDa band according to NP_000016.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-3 Adrenergic R/ADRB3 Antibody

• ADRB3
• ADRB3R
• adrenergic, beta-3-, receptor
• B3AR
• beta-3 Adrenergic R
• beta-3 adrenergic receptor
• beta3 AdrenergicR
• beta-3 AdrenergicR
• Beta-3 adrenoceptor
• Beta-3 adrenoreceptor
• BETA3AR
• FLJ99960

Background

The ADRB3 gene product, beta-3-adrenergic receptor, is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-3 Adrenergic R/ADRB3 Antibody (NB100-93369)(1)

Publication using NB100-93369

• Pierola J, Barcelo A, de la Pena M et al. THE Trp64Arg polymorphism of the beta (3) adrenergic receptor is associated with increased body mass index in patients with sleep apnoea. Eur Respir J 2007-07-11 [PMID: 17626108]

Bioinformatics

• Gene Symbol: ADRB3
• Entrez:
  • 155(Human)
• Uniprot:
  • NP_000016.1(Human)