beta-3 Adrenergic R/ADRB3 Antibody (493324) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human beta -3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta -3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

beta -3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta -3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 493324
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

beta-3 Adrenergic R/ADRB3 Antibody (493324) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived transfected with human beta -3 Adrenergic R/ADRB3
  Met1-Ser408
  Accession # P13945
• Specificity: Stains human beta -3 Adrenergic R/ADRB3 transfectants but not irrelevent transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-3 Adrenergic R/ADRB3 Antibody (493324) [Unconjugated]

• ADRB3
• ADRB3R
• adrenergic, beta-3-, receptor
• B3AR
• beta-3 Adrenergic R
• beta-3 adrenergic receptor
• beta3 AdrenergicR
• beta-3 AdrenergicR
• Beta-3 adrenoceptor
• Beta-3 adrenoreceptor
• BETA3AR
• FLJ99960

Background

 ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-3 Adrenergic R/ADRB3 Antibody (MAB9419)(1)

Publication using MAB9419

• G Schena, M Carmosino, S Chiurlia, L Onuchic, M Mastropasq, E Maiorano, FP Schena, MJ Caplan beta3 adrenergic receptor as potential therapeutic target in ADPKD Physiological Reports, 2021-10-01;9(20):e15058. 2021-10-01 [PMID: 34676684] (IHC, Human)

Bioinformatics

• Uniprot:
  • P13945()