Home » beta 2-Microglobulin » beta 2-Microglobulin Antibodies » beta 2-Microglobulin Antibody (SPM617) [DyLight 550]

beta 2-Microglobulin Antibody (SPM617) [DyLight 550]

 Submit a Review

Images

 
There are currently no images for beta 2-Microglobulin Antibody (NBP2-47703R).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
SPM617
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47703AF350
Alexa Fluor 405
NBP2-47703AF405
Alexa Fluor 488
NBP2-47703AF488
Alexa Fluor 532
NBP2-47703AF532
Alexa Fluor 594
NBP2-47703AF594
Alexa Fluor 647
NBP2-47703AF647
Alexa Fluor 700
NBP2-47703AF700
Alexa Fluor 750
NBP2-47703AF750
Allophycocyanin
NBP2-47703APC
Allophycocyanin/Cy7
NBP2-47703APCCY7
Biotin
NBP2-47703B
DyLight 350
NBP2-47703UV
DyLight 405
NBP2-47703V
DyLight 488
NBP2-47703G
DyLight 594
NBP2-47703DL594
DyLight 650
NBP2-47703C
DyLight 680
NBP2-47703FR
DyLight 755
NBP2-47703IR
FITC
NBP2-47703F
HRP
NBP2-47703H
Janelia Fluor 549
NBP2-47703JF549
Janelia Fluor 646
NBP2-47703JF646
PE
NBP2-47703PE
PE/Atto594
NBP2-47703PEATT594
PE/Cy5.5
NBP2-47703PECY55
PE/Cy7
NBP2-47703PECY7
PerCP
NBP2-47703PCP
Unconjugated
NBP2-44468-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44468-0.1mg
Azide and BSA Free
NBP2-47703-0.1mg
IHC-Prediluted
NBP2-48328
100% Guarantee on every product

beta 2-Microglobulin Antibody (SPM617) [DyLight 550] Summary

Immunogen
Full length recombinant human B2M protein
Localization
Cell Surface and Extracellular (Secreted)
Marker
Renal Failure & Tumor Marker
Specificity
Beta2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. Beta2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta 2-Microglobulin Antibody (SPM617) [DyLight 550]

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Albumin Antibody
NB600-41532
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.
(39 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
GAPDH Antibody
(1D4)
NB300-221
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
(18 Reviews)
(327 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single-Cell Western
beta-Actin
Antibody (AC-15)
NB600-501
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
(25 Reviews)
(443 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
Actin Gamma 1
Antibody
NB600-533
Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (http://neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
(6 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
HPRT Antibody
NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(35 Reviews)
(649 Publications)
Species: Hu
C-Reactive
Protein/CRP [HRP]
DCRP00
(3 Reviews)
(83 Publications)
Species: Hu
TATA binding
protein TBP Antibody ...
NBP2-59208
Western Blot: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - Whole cell extracts (40 ug) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against TBP diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.
Chromatin Immunoprecipitation Sequencing: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - IP'd DNA was analyzed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (A). The IP'd DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.
Chromatin Immunoprecipitation: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - ChIP results obtained with the antibody against TBP. ChIP assays were performed using U2OS cells, the antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.
Species: Hu, Mu
Applications: WB, ChIP, CHIP-SEQ, KD
IL-6 [HRP]
D6050
(26 Reviews)
(474 Publications)
Species: Hu
EIF3B Antibody
NBP2-24571
Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.
Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P

alpha-N-acetylglucosam ...
NBP1-31555
Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:250. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Paraffin-embedded mouse pancreas. NAGLU antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
Recombinant Human
NAG GST (N-Term) Prot ...
H00051594-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
GM-CSF
[Unconjugated]
7954-GM/CF
GM-CSF [Unconjugated]
(1 Publication)
Species: Hu
SCN7A Antibody
NB100-81029
Immunocytochemistry/Immunofluorescence: SCN7A Antibody [NB100-81029] - Staining of sodium channel protein type 7 subunit alpha in human sperm cells.
Immunohistochemistry-Paraffin: SCN7A Antibody [NB100-81029] - Staining of normal human heart tissue formalin fixed and paraffin embedded.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
HMBS Antibody
NBP1-87329
Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: HMBS Antibody [NBP1-87329] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Western Blot: HMBS Antibody [NBP1-87329] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Cystatin C [HRP]
MSCTC0
(21 Publications)
Species: Mu, Rt
RAPGEF5 Antibody
NBP2-57362
Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.
Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [NBP2-57362] - Staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.
Species: Hu
Applications: WB, ICC/IF
MHC Class I
Antibody (OX18)
NB120-6405
Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
(32 Publications)
Species: Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC

Publications for beta 2-Microglobulin Antibody (NBP2-47703R) (0)

There are no publications for beta 2-Microglobulin Antibody (NBP2-47703R).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta 2-Microglobulin Antibody (NBP2-47703R) (0)

There are no reviews for beta 2-Microglobulin Antibody (NBP2-47703R). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for beta 2-Microglobulin Antibody (NBP2-47703R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47703AF350
Alexa Fluor 405 NBP2-47703AF405
Alexa Fluor 488 NBP2-47703AF488
Alexa Fluor 532 NBP2-47703AF532
Alexa Fluor 594 NBP2-47703AF594
Alexa Fluor 647 NBP2-47703AF647
Alexa Fluor 700 NBP2-47703AF700
Alexa Fluor 750 NBP2-47703AF750
Allophycocyanin NBP2-47703APC
Allophycocyanin/Cy7 NBP2-47703APCCY7
Biotin NBP2-47703B
DyLight 350 NBP2-47703UV
DyLight 405 NBP2-47703V
DyLight 488 NBP2-47703G
DyLight 550 NBP2-47703R
DyLight 594 NBP2-47703DL594
DyLight 650 NBP2-47703C
DyLight 680 NBP2-47703FR
DyLight 755 NBP2-47703IR
FITC NBP2-47703F
HRP NBP2-47703H
Janelia Fluor 549 NBP2-47703JF549
Janelia Fluor 646 NBP2-47703JF646
PE NBP2-47703PE
PE/Atto594 NBP2-47703PEATT594
PE/Cy5.5 NBP2-47703PECY55
PE/Cy7 NBP2-47703PECY7
PerCP NBP2-47703PCP
Learn about Custom Antibody Labeling

Additional beta 2-Microglobulin Products

Bioinformatics Tool for beta 2-Microglobulin Antibody (NBP2-47703R)

Discover related pathways, diseases and genes to beta 2-Microglobulin Antibody (NBP2-47703R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta 2-Microglobulin Antibody (NBP2-47703R)

Discover more about diseases related to beta 2-Microglobulin Antibody (NBP2-47703R).
 

Pathways for beta 2-Microglobulin Antibody (NBP2-47703R)

View related products by pathway.

PTMs for beta 2-Microglobulin Antibody (NBP2-47703R)

Learn more about PTMs related to beta 2-Microglobulin Antibody (NBP2-47703R).
 

Research Areas for beta 2-Microglobulin Antibody (NBP2-47703R)

Find related products by research area.

Blogs on beta 2-Microglobulin

There are no specific blogs for beta 2-Microglobulin, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
beta 2-Microglobulin [Biotin]
beta 2-Microglobulin [Biotin]
KGE019

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our beta 2-Microglobulin Antibody (SPM617) [DyLight 550] and receive a gift card or discount.


Bioinformatics

Gene Symbol B2M