beta 2-Microglobulin Antibody (rB2M/961)

Flow Cytometric Analysis of PFA-fixed HeLa cells using beta 2-Microglobulin Antibody (rB2M/961) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta 2-Microglobulin Antibody (rB2M/961).

Western Blot Analysis of human kidney tissue lysates using beta 2-Microglobulin Antibody (rB2M/961).

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with beta 2-Microglobulin Antibody (rB2M/961).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with beta 2-Microglobulin Antibody (rB2M/961).

Analysis of Protein Array containing more than 19,000 full-length human proteins using beta 2-Microglobulin Antibody (rB2M/961).

• Reactivity: Hu, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: rB2M/961
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

beta 2-Microglobulin Antibody (rB2M/961) Summary

• Description: Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human full-length B2M protein (Uniprot: P61769)
• Localization: Cell surface, Secreted
• Source: HEK293
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B2M
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for beta 2-Microglobulin Antibody (rB2M/961)

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M