beta 2-Microglobulin Antibody (C21.48A1)

Western Blot: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Western Blot Analysis of THP-1 and Raji cell lysate using beta 2-Microglobulin Antibody (C21.48A1).

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (C21.48A1) followed by goat anti-Mouse IgG-CF488 (Green).

Flow Cytometry: beta 2-Microglobulin Antibody (C21.48A1) [NBP2-44470] - Flow Cytometric Analysis of PFA-fixed HeLa cells using beta 2-Microglobulin Antibody (C21.48A1)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

• Reactivity: Hu, Pm
• Applications: WB, Flow, ICC/IF, ICC/IF
• Clone: C21.48A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

beta 2-Microglobulin Antibody (C21.48A1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11404)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Soluble beta 2-Microglobulin (Uniprot: P61769)
• Localization: Cell Surface and Extracellular (Secreted)
• Marker: Renal Failure & Tumor Marker
• Specificity: The antibody recognizes the hidden determinant of beta-2 microglobulin (i.e. binding to its determinant is available only when the chain is separated from the HLA heavy chain. Beta-2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkins lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. Beta2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B2M
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Non-Human primates.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for beta 2-Microglobulin Antibody (C21.48A1)

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M
• Uniprot:
  • P61769()
  • P61769()