BCOR Antibody

Western Blot: BCOR Antibody [NB100-87005] - Detection of Human BCoR on HeLa whole cell lysate using NB100-87005. BCoR was also immunoprecipitated by rabbit anti-BCoR antibody NB100-87004.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

BCOR Antibody Summary

• Description: Novus Biologicals Rabbit BCOR Antibody (NB100-87005) is a polyclonal antibody validated for use in WB and IP. Anti-BCOR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1705 and 1755 of human BCL6 co-repressor using the numbering given in entry EAW59426.1 (GeneID 54880).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  BCOR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCOR Antibody

• ANOP2
• BCL6 corepressor
• BCL-6 corepressor
• BCL6 co-repressor
• BCL-6 interacting corepressor
• BCoR
• FLJ20285
• FLJ38041
• KIAA1575MGC71031
• MAA2
• MCOPS2
• MGC131961

Background

The protein encoded by this gene was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCOR Antibody (NB100-87005)(1)

Publication using NB100-87005

• Dietrich N, Lerdrup M, Landt E et al. REST-mediated recruitment of polycomb repressor complexes in mammalian cells. PLoS Genet 2012-01-01 [PMID: 22396653]

FAQs for BCOR Antibody (NB100-87005). (Showing 1 - 1 of 1 FAQ).

1. This antibody works fine with Hela nuclear extracts. The amino acid sequence of mentioned epitope has is an identity of 90% with mouse. Therefore, I wondered if the antibody can be used with mES nuclear extract?
   • We have not tested BCOR Antibody (NB100-87005) with mouse samples, and so cannot guarantee its use with this species. However, I agree that the sequence similarity between the immunogen sequence and the equivalent section of the mouse protein is high, indicating that the antibody is very likely to cross-react with mouse BCOR; normally our lab recommends using the antibody if the homology is 85% or higher. If you do go ahead and test NB100-87005 with mouse cells, you would be eligible for our Innovator's Reward, where in return for completing an online review with image, detailing your positive or negative results, you would receive a discount voucher for the purchase price of the reviewed product - please see here for further details: Innovator's Reward program. Alternatively, NBP1-68235 is a BCOR antibody that is guaranteed for mouse, although it isn't validated in immunoprecipitation, so if you used the antibody this particular application, again you would not be covered by our guarantee but you could apply for the Innovator's Reward.

Bioinformatics

• Gene Symbol: BCOR
• Entrez:
  • 54880(Human)
• Uniprot:
  • Q6W2J9(Human)