Bcl3 Antibody

Western Blot: Bcl3 Antibody [NB100-56375] - Analysis of Bcl-3 in Jurkat cells using this antibody at 0.5 ug/ml.

Product Discontinued

Bcl3 Antibody Summary

• Immunogen: This antibody was made against synthetic peptides corresponding to amino acids 2-13 and 107-120 of human Bcl-3. It will cross-react with mouse and rat, based on BLAST.
• Specificity: In Jurkat, a 45 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1 ug/ml
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Bcl3 Antibody

• B-cell CLL/lymphoma 3
• B-cell leukemia/lymphoma 3
• B-cell lymphoma 3 protein
• B-cell lymphoma 3-encoded protein
• BCL-3
• BCL4
• chronic lymphatic leukemia protein
• D19S37
• Proto-oncogene BCL3

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). The proto-oncoprotein Bcl-3 is a member of the IkB family and is present predominantly in the nucleus. IkBa and Bcl-3 are similar in the central ankyrin repeat domains, where as they differ at the N- and-C terminus. These differences may explain the differential binding of IkBa and Bcl-3. Two molecules of Bcl-3 can associate with NF-kB, whereas only one molecule of IkBa bind to NF-kB. BCL-3 mRNA and protein expression are induced by NF-kB-activating agents. Down-regulation of BCL-3 induction results in prolonged, enhanced NF-kB binding and increased NF-kB-dependent transcription. Several cytokines, such as, TNFa or IL-1b, and mitogens as phorbol ester (PMA) trigger rapid formation of Bcl-3-p50 complexes with the same kinetics as activation of p50-p65 complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL3
• Entrez:
  • 602(Human)
• Uniprot:
  • P20749(Human)