BCKDK Antibody - BSA Free

Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.

Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.

Western Blot: BCKDK Antibody [NBP2-15553] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: BCKDK Antibody [NBP2-15553] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] (NBP2-15553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BCKDK Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCKDK Antibody - BSA Free (NBP2-15553) is a polyclonal antibody validated for use in IHC and WB. Anti-BCKDK Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BCKDK. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Predicted Species: Bovine (98%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCKDK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BCKDK Antibody - BSA Free

• BCKDHKIN
• branched chain alpha-ketoacid dehydrogenase kinase
• branched chain ketoacid dehydrogenase kinase
• EC 2.7.11
• EC 2.7.11.4
• mitochondrial

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for BCKDK Antibody (NBP2-15553)(2)

Publications using NBP2-15553

• Wada E, Kobayashi M, Kohno D et al. Disordered branched chain amino acid catabolism in pancreatic islets is associated with postprandial hypersecretion of glucagon in diabetic mice: Postprandial hypersecretion of glucagon in diabetes The Journal of nutritional biochemistry 2021-06-28 [PMID: 34197915] (WB, Mouse)
• Shao D, Villet O, Zhang Z et al. Glucose promotes cell growth by suppressing branched-chain amino acid degradation. Nat Commun. 2018-07-26 [PMID: 30050148] (WB, Human)

Bioinformatics

• Gene Symbol: BCKDK
• Uniprot:
  • O14874(Human)