BCKDK Antibody (HL2710)

BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.BCKDK stained by BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL2710
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BCKDK Antibody (HL2710) Summary

• Description: Novus Biologicals Rabbit BCKDK Antibody (HL2710) (NBP3-48724) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human BCKDK.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCKDK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for BCKDK Antibody (HL2710)

• BCKDHKIN
• branched chain alpha-ketoacid dehydrogenase kinase
• branched chain ketoacid dehydrogenase kinase
• EC 2.7.11
• EC 2.7.11.4
• mitochondrial

Background

BCKDK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCKDK