BCKDHA Recombinant Protein Summary
A recombinant protein with a His-tag corresponding to amino acids 46 to 445 of Human BCKDHA
Source: E. coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl
The purity of this protein is > 80% by SDS-PAGE. Molecular weight is 47.8kDa (421aa) confirmed by MALDI-TOF
Alternate Names for BCKDHA Recombinant Protein
- 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
- BCKDH E1-alpha
- branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease)
- branched chain keto acid dehydrogenase E1, alpha polypeptide
- Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
- EC 188.8.131.52,2-oxoisovalerate dehydrogenase (lipoamide)
BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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