Recombinant Human BCKDHA Protein

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SDS-Page: Recombinant Human BCKDHA Protein [NBP1-51018] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human BCKDHA Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 46-445 of Human BCKDHA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
BCKDHA
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human BCKDHA Protein

  • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
  • BCKDE1A
  • BCKDH E1-alpha
  • branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease)
  • branched chain keto acid dehydrogenase E1, alpha polypeptide
  • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
  • EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide)
  • FLJ45695
  • MSU
  • MSUD1
  • OVD1A

Background

BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol BCKDHA