BCAP31 Antibody (JG37-81)

Western Blot: BCAP31 Antibody (JG37-81) [NBP2-75416] - Analysis of BAP31 on different lysates using anti-BAP31 antibody at 1/1,000 dilution.Positive control:Lane 1: A431 Lane 2: SK-Br-3

Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (JG37-81) [NBP2-75416] - Staining BAP31 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BCAP31 Antibody (JG37-81) [NBP2-75416] - Analysis of paraffin-embedded mouse testis tissue using anti-BAP31 antibody. Counter stained with hematoxylin.

Flow Cytometry: BCAP31 Antibody (JG37-81) [NBP2-75416] - Analysis of PC-3M cells with BAP31 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (JG37-81) [NBP2-75416] - Staining BAP31 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (JG37-81) [NBP2-75416] - Staining BAP31 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BCAP31 Antibody (JG37-81) [NBP2-75416] - Analysis of paraffin-embedded human liver tissue using anti-BAP31 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BCAP31 Antibody (JG37-81) [NBP2-75416] - Analysis of paraffin-embedded human colon cancer tissue using anti-BAP31 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BCAP31 Antibody (JG37-81) [NBP2-75416] - Analysis of paraffin-embedded human kidney tissue using anti-BAP31 antibody. Counter stained with hematoxylin.

Product Discontinued

BCAP31 Antibody (JG37-81) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human BCAP31 aa 81-220 / 246. (SwissProt: P51572 Human; SwissProt: Q61335 Mouse)
• Localization: Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCAP31
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BCAP31 Antibody (JG37-81)

• 6C6-Ag
• BAP31
• Bap31,6C6-AG
• BAP31DXS1357ECDM
• BCAP31
• B-cell receptor-associated protein 31
• BCR-associated protein 31
• BCR-associated protein Bap31,6C6-AG tumor-associated antigen
• p28 Bap31,6C6-Ag
• p28
• Protein CDM

Background

BAP31 (B Cell Receptor Associated Protein, 28 kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo and hetero oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL2/BCLXL associated protein. In the C terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCAP31