BCAP31 Antibody (CC-1)

Product Discontinued

BCAP31 Antibody (CC-1) Summary

• Immunogen: Solubilized membranes of the IMR-32 human neuroblastoma cell line.
• Specificity: Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, Bovine and Hamster BAP31.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: BCAP31
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:10000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000-1:6000
• Application Notes: This antibody is useful in ELISA (capture) and Immunoprecipitation. Works on endogenous for Immunocytochemistry, Immunohistochemistry-Frozen and Western Blot.

Reactivity Notes

Human, Primate, Bovine and Hamster. Does not recognize mouse and rat BAP31.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for BCAP31 Antibody (CC-1)

• 6C6-Ag
• BAP31
• Bap31,6C6-AG
• BAP31DXS1357ECDM
• BCAP31
• B-cell receptor-associated protein 31
• BCR-associated protein 31
• BCR-associated protein Bap31,6C6-AG tumor-associated antigen
• p28 Bap31,6C6-Ag
• p28
• Protein CDM

Background

BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca2+-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAP31 Antibody (NBP1-97439)(3)

Publications using NBP1-97439

• JJ Ladasky et al. Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules. J. Immunol. 177, 6172. 2006-01-01 [PMID: 17056546]
• K Zen et al. Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils. J. Biol. Chem. 279, 44924. 2004-01-01 [PMID: 15294914]
• HA Manley VA Lennon. Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells. J. Histochem. Cytochem. 49, 1235. 2011-01-01 [PMID: 11561007]

Bioinformatics

• Gene Symbol: BCAP31