BATF3 Antibody (841702) [Unconjugated]

Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c PE-conjugated Monoclonal Antibody (FAB1777P) and either (A) Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437) or (B) Mouse IgG2BFlow Cytometry Isotype Control (MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Jurkat cells were stained with Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 841702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BATF3 Antibody (841702) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human BATF3
  Met1-Arg127
  Accession # Q9NR55
• Specificity: Detects human BATF3 in ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BATF3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BATF3 Antibody (841702) [Unconjugated]

• basic leucine zipper transcription factor, ATF-like 3
• basic leucine zipper transcriptional factor ATF-like 3
• BATF3
• B-ATF-3
• FLJ36352
• FLJ37535,21 kDa small nuclear factor isolated from T-cells
• JDP1
• Jun dimerization protein 1
• Jun dimerization protein p21SNFT
• JUNDM1
• SNFT
• SNFT21-kD small nuclear factor isolated from T cells

Background

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c⁺), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BATF3 Antibody (MAB7437)(1)

Publication using MAB7437

• S Sirvent, AF Vallejo, J Davies, K Clayton, Z Wu, J Woo, J Riddell, VK Chaudhri, P Stumpf, LA Nazlamova, G Wheway, M Rose-Zeril, J West, M Pujato, X Chen, CH Woelk, B MacArthur, M Ardern-Jon, PS Friedmann, MT Weirauch, H Singh, ME Polak Genomic programming of IRF4-expressing human Langerhans cells Nat Commun, 2020-01-16;11(1):313. 2020-01-16 [PMID: 31949143] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: BATF3
• Uniprot:
  • Q9NR55()