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BATF3 Antibody (841702) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
841702
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
FAB7437V-100UG
Alexa Fluor 488
IC7437G
Alexa Fluor 594
FAB7437T-100UG
Alexa Fluor 647
FAB7437R-100UG
Alexa Fluor 700
FAB7437N-100UG
Alexa Fluor 750
FAB7437S-100UG
Unconjugated
MAB7437
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB7437
Carrier Free
MAB7437
Carrier Free
IC7437G
Carrier Free
MAB7437-SP
Carrier Free
FAB7437V-100UG
Carrier Free
FAB7437T-100UG
Carrier Free
FAB7437S-100UG
Carrier Free
FAB7437R-100UG
Carrier Free
FAB7437N-100UG
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BATF3 Antibody (841702) [Alexa Fluor® 350] Summary

Immunogen
E. coli-derived recombinant human BATF3
Met1-Arg127
Accession # Q9NR55
Specificity
Detects human BATF3 in ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BATF3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BATF3 Antibody (841702) [Alexa Fluor® 350]

  • basic leucine zipper transcription factor, ATF-like 3
  • basic leucine zipper transcriptional factor ATF-like 3
  • BATF3
  • B-ATF-3
  • FLJ36352
  • FLJ37535,21 kDa small nuclear factor isolated from T-cells
  • JDP1
  • Jun dimerization protein 1
  • Jun dimerization protein p21SNFT
  • JUNDM1
  • SNFT
  • SNFT21-kD small nuclear factor isolated from T cells

Background

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.
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Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
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TLR3 Antibody
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JunD [p Ser255]
Antibody (JJ08-21)
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NBP1-89544
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Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining in human fallopian tube and skeletal muscle tissues. Corresponding JUNB RNA-seq data are presented for the same tissues.
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Applications: WB, ICC/IF, IHC, IHC-P
Flt-3/Flk-2/CD135
Antibody (7E8.2C8)
NBP2-42210
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.
Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis
Species: Hu
Applications: WB, Flow, IHC, IHC-P
FosB/G0S3 Antibody
(83B1138)
NB100-56530
Western Blot: FosB/G0S3 Antibody (83B1138) [NB100-56530] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB.
Species: Hu
Applications: WB
GM-CSF
[Unconjugated]
7954-GM/CF
GM-CSF [Unconjugated]
(1 Publication)
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CLEC9a Antibody
(14N8D7)
NBP2-27087
Western Blot: CLEC9a Antibody (14N8D7) [NBP2-27087] - Analysis of A) HCT-116 and B) MCF-7 lysate using this antibody at 1 ug/ml.
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Applications: WB
Langerin/CD207
Antibody (929F3.01)
DDX0362P-100
Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Langerin Antibody (929F3.01) [DDX0362P-100] - IF staining on isolated skin-isolated porcine Langerhans cells.
Immunohistochemistry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Epidermal LC are dispensable for betaGal-specific immunity but down-regulate OVA-specific T cell functions. LangDTR or B6 WT mice were injected once with 1ug DT. Epidermal sheets were stained for langerin expression 3 wks later. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0128722), licensed under a CC-BY licence.
Flow Cytometry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Cells derived from culture of porcine skin stained permeabilized (D) or not (C) with 929F3
(69 Publications)
Species: Hu, Mu, Rt, Po, Eq
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
TCF4 Antibody (1G4)
H00006925-M03
Western Blot: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of TCF4 expression in K-562 (Cat # L009V1).
Immunocytochemistry/Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of monoclonal antibody to TCF4 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: TCF4 Antibody (1G4) [H00006925-M03] - Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA

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FAQs for BATF3 Antibody (FAB7437U) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB7437U
Alexa Fluor 405 FAB7437V
Alexa Fluor 488 IC7437G
Alexa Fluor 594 FAB7437T
Alexa Fluor 647 FAB7437R
Alexa Fluor 700 FAB7437N
Alexa Fluor 750 FAB7437S
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol BATF3
Uniprot