CLEC9a Antibody (14N8D7)

Western Blot: CLEC9a Antibody (14N8D7) [NBP2-27087] - Analysis of A) HCT-116 and B) MCF-7 lysate using this antibody at 1 ug/ml.

• Reactivity: Hu, Pm, RM
• Applications: WB
• Clone: 14N8D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CLEC9a Antibody (14N8D7) Summary

• Immunogen: A portion of amino acids 50-130 of human CLEC9A was used as the immunogen for the antibody.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC9A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3ug/ml
• Application Notes: CLEC9A is a glycoprotein visualized by western blot between ~25-45 kD.

Reactivity Notes

Orangutan, Chimp (100%), Canine, Panda, Equine (73%), Bovine (73%), Mouse (60%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CLEC9a Antibody (14N8D7)

• CD370
• CLEC9a
• C-type lectin domain family 9 member A
• C-type lectin domain family 9, member A
• DNGR1
• HEEE9341
• UNQ9341

Background

CLEC9A is a group V CTLR (C-type lectin-like receptor) that functions as an activation receptor and is expressed on myeloid lineage cellsThis protein is also expressed at the cell surface and acts as glycosylated dimers which mediate endocytosis, but not phagocytosis (Poulin LF et al, 2010). It facilitates antigen uptake and presentation, and may provide a suitable target for antibody-mediated antigen delivery. CLEC9A is important for conveying information from necrotic cells to T-cells and defines a pathway by which adaptive immune responses can be initiated in the absence of infection (Huysamen C et al, 2008). It recruits Syk to mediate sensing of necrosis by the principal DC subset involved in regulating cross priming to cell-associated antigens (Sancho D et al, 2009). Signaling via this kinase occurs through a novel pathway involving CARD9 which induce a variety of cellular responses including the induction of cytokines (such as TNF, IL-6, IL-10, IL-23, and IL-2), the respiratory burst, and the production of Arachidonic acid (Caminschi I et al, 2008). In addition, it also induces TH17 (T-helper type 17) adaptive responses in vivo. Expression of CLEC9A is highly restricted in peripheral blood, being detected only on BDCA3+ DCs and on a small subset of CD14+ CD16- monocytes. CLEC9A serves as a new marker to distinguish subtypes of DCs. Targeting antigens to DCs with antibodies to CLEC9A is a promising strategy to enhance the efficiency of vaccines, even in the absence of adjuvants (Sancho D et al, 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC9A
• Uniprot:
  • Q6UXN8()