BARD1 Antibody - BSA Free

Immunohistochemistry-Paraffin: BARD1 Antibody [NBP3-13065] - BARD1 antibody detects BARD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BARD1 stained by BARD1 antibody (NBP3-13065) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: BARD1 Antibody [NBP3-13065] - A431 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BARD1 antibody (NBP3-13065) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

BARD1 antibody detects BARD1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BARD1 protein stained by BARD1 antibody (NBP3-13065) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BARD1 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BARD1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BARD1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for BARD1 Antibody - BSA Free

• BARD-1
• BRCA1 associated RING domain 1
• BRCA1-associated RING domain gene 1
• BRCA1-associated RING domain protein 1
• EC 6.3.2.-

Background

BRCA1-Associated RING Domain gene 1 (BARD1) functions as a tumor suppressor, especially in breast and ovarian cancers. It plays a major role in cancer suppression when it forms a heterodimer with BRCA1. It also plays a role in mediating apoptotic stress and p53-dependent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BARD1