BAI2 Antibody (2528B) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human BAI2. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BAI2 at approximately 172 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with (A) human BAI2 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human BAI2 Monoclonal Antibody (Catalog # MAB9338) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 2528B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BAI2 Antibody (2528B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell, HEK293-derived human BAI2
  Phe21-Asp911
  Accession # O60241-3
• Specificity: Detects human BAI2 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAI2 Antibody (2528B) [Unconjugated]

• BAI2
• brain-specific angiogenesis inhibitor 2
• Brain-specific angiongenesis inhibitor-2

Background

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

1. Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.
2. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
3. Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.
4. Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.
5. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
6. Jeong, B.C. et al. (2016) FEBS Lett 580:669.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAI2 Antibody (MAB9338)(1)

Publication using MAB9338

• Meyer, CM;Vafaeva, O;Low, H;Speca, DJ;Díaz, E; Regulation of hippocampal excitatory synapse development by the adhesion G-protein coupled receptor Brain-specific angiogenesis inhibitor 2 (BAI2/ADGRB2) bioRxiv : the preprint server for biology 2025-02-03 [PMID: 39975290] (Immunocytochemistry, Mouse)

Bioinformatics

• Uniprot:
  • O60241-3()