BAFFR/TNFRSF13C Antibody (2403C) [Unconjugated]

Peripheral blood mononuclear cells (PBMCs) were stained with either (A) Rabbit Anti-Human BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1162) or (B) Rabbit IgG Control Antibody (Catalog # MAB1050) followed by APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.

BAFF R/TNFRSF13C was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1162) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 2403C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BAFFR/TNFRSF13C Antibody (2403C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line, NS0-derived human BAFF R/TNFRSF13C
  Ser7-Ala71
  Accession # Q96RJ3
• Specificity: Detects human BAFF R/TNFRSF13C in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFFR/TNFRSF13C Antibody (2403C) [Unconjugated]

• BAFF R
• BAFFR
• BAFFR;BAFF-R;BROMIX;CD268;CVID4;prolixin;Tumor necrosis factor receptor superfamily member 13C
• BR3
• CD268
• TNFRSF13C

Background

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4⁺ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.

1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
4. Thompson, J.S. et al. (2001) Science 14:2108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for BAFFR/TNFRSF13C Antibody (MAB1162) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lydia Taverne IHC-P Human 12/16/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human spleen, FFPE
• Species: Human
• Lot: IRN0217041

Comments

• Comments: Citrate buffer (pH 6) antigen unmasking. Antibody was used at a 1:50 concentration and detected with anti-rabbit AF546 (red fluor) secondary Ab.

Bioinformatics

• Uniprot:
  • Q96RJ3()