BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free

Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: Buffy-2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free Summary

• Immunogen: Recombinant human soluble BAFF (aa 83-285).
• Specificity: Recognizes membrane-bound and soluble human and mouse (weak) BAFF.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF13B
• Purity: Thiophilic Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot

Reactivity Notes

There is a weak corss-reactivity to Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Thiophilic Chromatography

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (Buffy-2) - BSA Free

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

TNFSF13B recognizes membrane bound and soluble human and mouse(weak) BAFF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAFF/BLyS/TNFSF13B Antibody (NBP1-97622)(8)

Publications using NBP1-97622

• Pelekanou Vassiliki, Kampa Marilena, Kafousi Maria et al. Expression of TNF-superfamily members BAFF and APRIL in breast cancer: immunohistochemical study in 52 invasive ductal breast carcinomas. BMC Cancer. 2008-01-01 [PMID: 18366696]
• Chu VT, Enghard P, Riemekasten G et al. In vitro and in vivo activation induces BAFF and APRIL expression in B cells. J Immunol. 2007-11-01 [PMID: 17947668]
• Ogden CA, Pound JD, Batth BK et al. Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma. J Immunol. 2005-03-01 [PMID: 15728515]
• Novak AJ, Grote DM, Stenson M et al. Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation with disease activity and patient outcome. Blood. 2004-10-01 [PMID: 15251985]
• He B, Chadburn A, Jou E et al. Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL. J Immunol. 2004-03-01 [PMID: 14978135]
• Huard B, Arlettaz L, Ambrose C et al. BAFF production by antigen-presenting cells provides T cell co-stimulation. Int Immunol. 2004-03-01 [PMID: 14978020]
• Novak AJ, Darce JR, Arendt BK et al. Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival. Blood. 2004-01-01 [PMID: 14512299]
• Groom J, Kalled SL, Cutler AH et al. Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome. J Clin Invest. 2002-01-01 [PMID: 11781351]

Review for BAFF/BLyS/TNFSF13B Antibody (NBP1-97622) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Masahiro Hirata IHC-P Human 01/21/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human
• Lot: 11051428

Comments

• Comments: Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. rabbit ant-rat IgG+M as a linker antibody
  pretreatment: basic solution (BOND ER2)

Bioinformatics

• Gene Symbol: TNFSF13B