BAFF/BLyS/TNFSF13B Antibody (121808) [Unconjugated]

Mouse Splenocytes were stained with Rat Anti-Mouse BAFF/BLyS/TNFSF13B Monoclonal Antibody (Catalog # MAB1357, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 121808
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

BAFF/BLyS/TNFSF13B Antibody (121808) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BAFF
  Ala127-Leu309
  Accession # Q9WU72
• Specificity: Detects mouse BAFF in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) 4-1BB Ligand, rmEDA, rmFas Ligand, rmOX40 Ligand, rmTNF-alpha , rmTRANCE, rmTWEAK, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhTNF-alpha , rhTRAIL, rhVEGI, recombinant canine, cotton rat, equine, feline, porcine, or rat TNF-alpha .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF13B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (121808) [Unconjugated]

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor(BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha , IFN-beta , LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
4. Liu, Y. et al. (2002) Cell 108:383. 
5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. 
6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
7. Thompson, J.S. et al. (2001) Science 293:2108.
8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAFF/BLyS/TNFSF13B Antibody (MAB1357)(12)

Publications using MAB1357

• Grace S. Pham, Lei A. Wang, Keisa W. Mathis Pharmacological potentiation of the efferent vagus nerve attenuates blood pressure and renal injury in a murine model of systemic lupus erythematosus American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 12/1/2018 [PMID: 30332305]
• Grace S. Pham, Lei A. Wang, Keisa W. Mathis Pharmacological potentiation of the efferent vagus nerve attenuates blood pressure and renal injury in a murine model of systemic lupus erythematosus American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2018-12-01 [PMID: 30332305] (Western Blot, Mouse)
• T Matsushita, T Kobayashi, K Mizumaki, M Kano, T Sawada, M Tennichi, A Okamura, Y Hamaguchi, Y Iwakura, M Hasegawa, M Fujimoto, K Takehara BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance Sci Adv, 2018-07-11;4(7):eaas9944. 2018-07-11 [PMID: 30009261] (Flow Cytometry, Mouse)
• A De Riva, M Wållberg, F Ronchi, R Coulson, A Sage, L Thorne, I Goodfellow, KD McCoy, M Azuma, A Cooke, R Busch Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment PLoS ONE, 2017-08-03;12(8):e0181964. 2017-08-03 [PMID: 28771521] (ELISA Development (Capture), Mouse)
• Cremasco , Viviana, Woodruff , Matthew, Onder , Lucas, Cupovic , Jovana, Nieves-Bonilla , Janice M, Schildberg , Frank A, Chang , Jonathan, Cremasco , Floriana, Harvey , Christop, Wucherpfennig , Kai, Ludewig , Burkhard, Carroll , Michael, Turley , Shannon B cell homeostasis and follicle confines are governed by fibroblastic reticular cells. Nat Immunol, 2014-08-24;15(10):973-81. 2014-08-24 [PMID: 25151489] (Flow Cytometry, Mouse)
• Yasuda K, Watkins A, Kochar G, Wilson G, Laskow B, Richez C, Bonegio R, Rifkin I Interferon regulatory factor-5 deficiency ameliorates disease severity in the MRL/lpr mouse model of lupus in the absence of a mutation in DOCK2. PLoS ONE, 2014-07-30;9(7):e103478. 2014-07-30 [PMID: 25076492] (ELISA Development (Capture), Mouse)
• Giordano D, Draves K, Li C, Hohl T, Clark E Nitric oxide regulates BAFF expression and T cell-independent antibody responses. J Immunol, 2014-06-20;193(3):1110-20. 2014-06-20 [PMID: 24951820] (Flow Cytometry, Mouse)
• Kanagavelu , Saravana, Termini , James M, Gupta , Sachin, Raffa , Francesc, Fuller , Katherin, Rivas , Yaelis, Philip , Sakhi, Kornbluth , Richard, Stone , Geoffrey HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity. PLoS ONE, 2014-02-28;9(2):e90100. 2014-02-28 [PMID: 24587225] (Western Blot, Human)
• Goenka , Radhika, Matthews , Andrew H, Zhang , Bochao, O'Neill , Patrick, Scholz , Jean L, Migone , Thi-Sau, Leonard , Warren J, Stohl , William, Hershberg , Uri, Cancro , Michael Local BLyS production by T follicular cells mediates retention of high affinity B cells during affinity maturation. J Exp Med, 2013-12-23;211(1):45-56. 2013-12-23 [PMID: 24367004] (Flow Cytometry, Mouse)
• Scapini P, Hu Y, Chu CL Myeloid cells, BAFF, and IFN-gamma establish an inflammatory loop that exacerbates autoimmunity in Lyn-deficient mice. J. Exp. Med., 2010-07-12;207(8):1757-73. 2010-07-12 [PMID: 20624892] (ELISA Development, Mouse)
• Parameswaran R, Ben David H, Sharabi A, Zinger H, Mozes E B-cell activating factor (BAFF) plays a role in the mechanism of action of a tolerogenic peptide that ameliorates lupus. Clin. Immunol., 2009-02-01;131(2):223-32. 2009-02-01 [PMID: 19188092] (Western Blot, Mouse)
• Wan S, Zhou Z, Duan B, Morel L Direct B cell stimulation by dendritic cells in a mouse model of lupus. Arthritis Rheum., 2008-06-01;8(0):1741-50. 2008-06-01 [PMID: 18512810] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TNFSF13B
• Uniprot:
  • Q9WU72()