BAFF/BLyS/TNFSF13B Antibody (121808) [Alexa Fluor® 594]

Images

 
There are currently no images for BAFF/BLyS/TNFSF13B Antibody (FAB1357T).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
121808
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

BAFF/BLyS/TNFSF13B Antibody (121808) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAFF
Ala127-Leu309
Accession # Q9WU72
Specificity
Detects mouse BAFF in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) 4-1BB Ligand, rmEDA, rmFas Ligand, rmOX40 Ligand, rmTNF-alpha, rmTRANCE, rmTWEAK, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhTNF-alpha, rhTRAIL, rhVEGI, recombinant canine, cotton rat, equine, feline, porcine, or rat TNF-alpha.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TNFSF13B
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (121808) [Alexa Fluor® 594]

  • ApoL related ligand TALL-1
  • B lymphocyte stimulator
  • BAFF
  • BAFFB-cell activating factor
  • B-cell-activating factor
  • BLyS
  • BLYSB-lymphocyte stimulator
  • CD257 antigen
  • CD257
  • Dendritic cell-derived TNF-like molecule
  • DTL
  • TALL1
  • TALL-1delta BAFF
  • TALL1Delta4 BAFF
  • THANK
  • TNF- and APOL-related leukocyte expressed ligand 1
  • TNF and ApoL-related leukocyte expressed ligand 1
  • TNF homolog that activates apoptosis
  • TNFSF13B
  • TNFSF20
  • tumor necrosis factor (ligand) superfamily, member 13b
  • tumor necrosis factor (ligand) superfamily, member 20
  • tumor necrosis factor ligand superfamily member 13B
  • tumor necrosis factor superfamily, member 13b
  • tumor necrosis factor-like protein ZTNF4
  • ZTNF4

Background

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor(BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
  4. Liu, Y. et al. (2002) Cell 108:383. 
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. 
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-97587
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-56516
Species: Hu, Mu
Applications: WB, IHC
AF1162
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
NBP1-97637
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
7270-IL/CF
Species: Hu
NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
AF4966
Species: Hu
Applications: WB, Simple Western, IHC
H00005478-M01
Species: Hu
Applications: WB, ELISA
6507-IL/CF
Species: Hu
AF5129
Species: Hu
Applications: WB
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, In vitro, CyTOF-ready
NBP1-85599
Species: Hu
Applications: WB, IHC, IHC-P
FAB1357T
Species: Mu
Applications: Flow

Publications for BAFF/BLyS/TNFSF13B Antibody (FAB1357T) (0)

There are no publications for BAFF/BLyS/TNFSF13B Antibody (FAB1357T).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BAFF/BLyS/TNFSF13B Antibody (FAB1357T) (0)

There are no reviews for BAFF/BLyS/TNFSF13B Antibody (FAB1357T). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for BAFF/BLyS/TNFSF13B Antibody (FAB1357T) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for BAFF/BLyS/TNFSF13B Antibody (FAB1357T)

Discover related pathways, diseases and genes to BAFF/BLyS/TNFSF13B Antibody (FAB1357T). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BAFF/BLyS/TNFSF13B Antibody (FAB1357T)

Discover more about diseases related to BAFF/BLyS/TNFSF13B Antibody (FAB1357T).
 

Pathways for BAFF/BLyS/TNFSF13B Antibody (FAB1357T)

View related products by pathway.

PTMs for BAFF/BLyS/TNFSF13B Antibody (FAB1357T)

Learn more about PTMs related to BAFF/BLyS/TNFSF13B Antibody (FAB1357T).

Blogs on BAFF/BLyS/TNFSF13B

There are no specific blogs for BAFF/BLyS/TNFSF13B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

BAFF/BLyS/TNFSF13B
7537-BF-025/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BAFF/BLyS/TNFSF13B Antibody (121808) [Alexa Fluor® 594] and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFSF13B
Uniprot