BAF53A Antibody

Images

 
Western Blot: BAF53A Antibody [NBP1-55378] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.
Western Blot: BAF53A Antibody [NBP1-55378] - Mouse WT brain and Rat brain, concentration 2ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BAF53A Antibody Summary

Immunogen
Synthetic peptides corresponding to ACTL6A (actin-like 6A) The peptide sequence was selected from the N terminal of ACTL6A)(50ug). Peptide sequence VDFPTAIGMVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTL6A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against ACTL6A and was validated on Western blot.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for BAF53A Antibody

  • actin-like 6A
  • actin-like protein 6A
  • actin-related protein 4,53 kDa BRG1-associated factor A
  • Actin-related protein Baf53a
  • Actl6
  • Arp4
  • arpNbeta
  • BAF complex 53 kDa subunit
  • Baf53a
  • BAF53AACTL6
  • BAF53MGC5382
  • BRG1-associated factor 53A
  • hArpN beta
  • INO80 complex subunit K
  • INO80KARPN-BETA

Background

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACTL6A
Uniprot