BACH1 Antibody (OTI4E11) - Azide and BSA Free

Western Blot: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BACH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BACH1.

Immunohistochemistry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody.

Flow Cytometry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BACH1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - Staining of paraffin-embedded Human tonsil using anti-BACH1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI4E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

BACH1 Antibody (OTI4E11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse BACH1 Antibody (OTI4E11) - Azide and BSA Free (NBP2-01904) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BACH1(NP_996749) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BACH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 81.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for BACH1 Antibody (OTI4E11) - Azide and BSA Free

• BACH1
• basic region leucine zipper transcriptional regulator BACH1
• BTB and CNC homolog 1
• BTB and CNC homology 1, basic leucine zipper transcription factor 1
• BTB
• HA2303
• transcription regulator protein BACH1
• transcription regulator protein10BACH-1

Background

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BACH1