BACH1 Antibody (OTI4E11)

Western Blot: BACH1 Antibody (4E11) [NBP2-01904] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BACH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BACH1.

Immunohistochemistry-Paraffin: BACH1 Antibody (4E11) [NBP2-01904] Staining of paraffin-embedded Human tonsil using anti-BACH1 mouse monoclonal antibody.

Flow Cytometry: BACH1 Antibody (4E11) [NBP2-01904] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BACH1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: BACH1 Antibody (4E11) [NBP2-01904] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: OTI4E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

BACH1 Antibody (OTI4E11) Summary

• Description: Novus Biologicals Mouse BACH1 Antibody (OTI4E11) (NBP2-01904) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-BACH1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BACH1(NP_996749) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BACH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 81.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BACH1 Antibody (OTI4E11)

• BACH1
• basic region leucine zipper transcriptional regulator BACH1
• BTB and CNC homolog 1
• BTB and CNC homology 1, basic leucine zipper transcription factor 1
• BTB
• HA2303
• transcription regulator protein BACH1
• transcription regulator protein10BACH-1

Background

This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BACH1 Antibody (NBP2-01904)(2)

Publications using NBP2-01904

• El-Deek H, Mohammed R, Ahmed A. Aberration of Nrf2-Bach1 pathway in colorectal carcinoma; role in carcinogenesis and tumor progression. Lipids. 2018-09-01 [PMID: 30597358] (IF/IHC, Human)
• Cha YJ, Lee JH, Han HH et al. MicroRNA alteration and putative target genes in high-grade prostatic intraepithelial neoplasia and prostate cancer: STAT3 and ZEB1 are upregulated during prostate carcinogenesis. Prostate 2016-07-01 [PMID: 27017949]

Bioinformatics

• Gene Symbol: BACH1
• Entrez:
  • 571(Human)