B7-H6 Antibody (1002910) [Unconjugated]

Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or transfected with cynomologus monkey B7-H6. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7-H6 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with Human B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with Cynomolgus Monkey B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Pm-Cm
• Applications: WB, Flow, CyTOF-ready
• Clone: 1002910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

B7-H6 Antibody (1002910) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived Cynomolgus Monkey B7‑H6
  Asp25-Ser262
• Specificity: Detects human and cynomolgus monkey B7‑H6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H6 Antibody (1002910) [Unconjugated]

• B7 homolog 6
• B7H6
• B7-H6
• DKFZp686I21167
• DKFZp686O24166
• NCR3LG1

Background

B7-H6 is a 55 kDa glycosylated member of the B7 family of immune costimulatory proteins (1, 2). Mature B7-H6 consists of an extracellular domain (ECD) that with one Ig-like V domain and one Ig-like C1 domain, a transmembrane segment, and a cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, cynomolgous B7-H6 shares 81%, 81%, 77% and 80% amino acid sequence identity with chimpanzee, orangutan, gibbon, and human B7-H6, respectively. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). B7-H6 is up-regulated on inflammatory monocytes and neutrophils, and a 30 kDa soluble fragment can be released by ADAM-10 or ADAM-17 mediated shedding (8, 9). The expression of B7-H6 on tumor cells correlates with cancer progression and patient's survival in human ovarian cancer (10).

1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:6223.
6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.
8. Matta, J. et al. (2013) Blood 122:394.
9. Schlecker, E. et al. (2014) Cancer Res. 74:3429.
10. Zhou, Y. et al. (2015) Int. J. Clin. Exp. Pathol. 8:9428.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.