B7-H4 Antibody (188) - BSA Free

Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug Anti-Mouse B7-H4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysis.

• Reactivity: Mu
• Applications: Flow
• Clone: 188
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free

B7-H4 Antibody (188) - BSA Free Summary

• Description: Novus Biologicals Rat B7-H4 Antibody (188) - BSA Free (NBP1-43266) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was Purified B7H4.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: VTCN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The 188 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for B7-H4 Antibody (188) - BSA Free

• B7h.5
• B7H4
• B7-H4
• B7H4T-cell costimulatory molecule B7x
• B7S1
• B7S1VCTN1
• B7x
• B7XPRO1291
• FLJ22418
• Immune costimulatory protein B7-H4
• Protein B7S1
• T cell costimulatory molecule B7x
• V-set domain containing T cell activation inhibitor 1
• V-set domain-containing T-cell activation inhibitor 1
• Vtcn1

Background

The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X. Cross reactivity of this antibody to other proteins has not been determined. B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. The ligand for B7-H4 has not been identified yet. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, and , suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together. 188 stains mouse B7-H4 transfected cells and not spleen cells. Exact expression pattern of B7-H4 has not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VTCN1