B7-H4 Antibody (188)

Images

 
Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
188
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

B7-H4 Antibody (188) Summary

Immunogen
The immunogen for this antibody was Purified B7H4.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
VTCN1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The 188 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B7-H4 Antibody (188)

  • B7h.5
  • B7H4
  • B7-H4
  • B7H4T-cell costimulatory molecule B7x
  • B7S1
  • B7S1VCTN1
  • B7x
  • B7XPRO1291
  • FLJ22418
  • Immune costimulatory protein B7-H4
  • Protein B7S1
  • T cell costimulatory molecule B7x
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain-containing T-cell activation inhibitor 1
  • Vtcn1

Background

The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X. Cross reactivity of this antibody to other proteins has not been determined. B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. The ligand for B7-H4 has not been identified yet. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, and, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together. 188 stains mouse B7-H4 transfected cells and not spleen cells. Exact expression pattern of B7-H4 has not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43266AF405
Alexa Fluor 488 Labeled NBP1-43266AF488
Alexa Fluor 647 Labeled NBP1-43266AF647
Alexa Fluor 700 Labeled NBP1-43266AF700
Allophycocyanin Labeled NBP1-43266APC
DyLight 350 Labeled NBP1-43266UV
DyLight 405 Labeled NBP1-43266V
DyLight 488 Labeled NBP1-43266G
DyLight 680 Labeled NBP1-43266FR
DyLight 755 Labeled NBP1-43266IR
PE Labeled NBP1-43266PE
PerCP Labeled NBP1-43266PCP

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Bioinformatics

Gene Symbol VTCN1
Entrez