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B7-H4 Antibody (188)

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Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug Anti-Mouse B7-H4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
188
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43266AF350
Alexa Fluor 405
NBP1-43266AF405
Alexa Fluor 488
NBP1-43266AF488
Alexa Fluor 532
NBP1-43266AF532
Alexa Fluor 594
NBP1-43266AF594
Alexa Fluor 647
NBP1-43266AF647
Alexa Fluor 700
NBP1-43266AF700
Alexa Fluor 750
NBP1-43266AF750
Allophycocyanin
NBP1-43266APC
Allophycocyanin/Cy7
NBP1-43266APCCY7
DyLight 350
NBP1-43266UV
DyLight 405
NBP1-43266V
DyLight 488
NBP1-43266G
DyLight 550
NBP1-43266R
DyLight 594
NBP1-43266DL594
DyLight 650
NBP1-43266C
DyLight 680
NBP1-43266FR
DyLight 755
NBP1-43266IR
Janelia Fluor 549
NBP1-43266JF549
Janelia Fluor 646
NBP1-43266JF646
PE
NBP1-43266PE
PE/Atto594
NBP1-43266PEATT594
PE/Cy5.5
NBP1-43266PECY55
PE/Cy7
NBP1-43266PECY7
PerCP
NBP1-43266PCP
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B7-H4 Antibody (188) Summary

Immunogen
The immunogen for this antibody was Purified B7H4.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
VTCN1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The 188 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B7-H4 Antibody (188)

  • B7h.5
  • B7H4
  • B7-H4
  • B7H4T-cell costimulatory molecule B7x
  • B7S1
  • B7S1VCTN1
  • B7x
  • B7XPRO1291
  • FLJ22418
  • Immune costimulatory protein B7-H4
  • Protein B7S1
  • T cell costimulatory molecule B7x
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain-containing T-cell activation inhibitor 1
  • Vtcn1

Background

The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X. Cross reactivity of this antibody to other proteins has not been determined. B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. The ligand for B7-H4 has not been identified yet. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, and, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together. 188 stains mouse B7-H4 transfected cells and not spleen cells. Exact expression pattern of B7-H4 has not been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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B7-H4 Antibody
(188)
NBP1-43266
B7-H4 Antibody (188)
Species: Mu
Applications: Flow

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43266AF350
Alexa Fluor 405 NBP1-43266AF405
Alexa Fluor 488 NBP1-43266AF488
Alexa Fluor 532 NBP1-43266AF532
Alexa Fluor 594 NBP1-43266AF594
Alexa Fluor 647 NBP1-43266AF647
Alexa Fluor 700 NBP1-43266AF700
Alexa Fluor 750 NBP1-43266AF750
Allophycocyanin NBP1-43266APC
Allophycocyanin/Cy7 NBP1-43266APCCY7
DyLight 350 NBP1-43266UV
DyLight 405 NBP1-43266V
DyLight 488 NBP1-43266G
DyLight 550 NBP1-43266R
DyLight 594 NBP1-43266DL594
DyLight 650 NBP1-43266C
DyLight 680 NBP1-43266FR
DyLight 755 NBP1-43266IR
Janelia Fluor 549 NBP1-43266JF549
Janelia Fluor 646 NBP1-43266JF646
PE NBP1-43266PE
PE/Atto594 NBP1-43266PEATT594
PE/Cy5.5 NBP1-43266PECY55
PE/Cy7 NBP1-43266PECY7
PerCP NBP1-43266PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol VTCN1
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