B7-H3/CD276 Antibody (185504) [Phycoerythrin]

Human monocyte-derived dendritic cells were stained with Mouse Anti-Human B7-H3 PE-conjugated Monoclonal Antibody (Catalog # FAB1027P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 PE-conjugated Monoclonal Antibody (Catalog # FAB1027P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 185504
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

B7-H3/CD276 Antibody (185504) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human B7‑H3
  Leu29-Pro245
  Accession # NP_079516
• Specificity: Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD276
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H3/CD276 Antibody (185504) [Phycoerythrin]

• B7H3
• B7-H3
• B7H34Ig-B7-H3
• B7-H3B7 homolog 3
• CD276 antigen
• CD276 molecule
• CD276
• Costimulatory molecule

Background

B7-H3 is a member of the B7 family of immune costimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-H3/CD276 Antibody (FAB1027P)(3)

Publications using FAB1027P

• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (ICC, Human)
• Yifei Wang, Xiangjun Tian, Wendong Zhang, Zhongting Zhang, Rossana Lazcano, Pooja Hingorani et al. Comprehensive Surfaceome Profiling to Identify and Validate Novel Cell-Surface Targets in Osteosarcoma Molecular Cancer Therapeutics 6/1/2022 [PMID: 35312779]
• J Theruvath, E Sotillo, CW Mount, CM Graef, A Delaidelli, S Heitzenede, L Labanieh, S Dhingra, A Leruste, RG Majzner, P Xu, S Mueller, DW Yecies, MA Finetti, D Williamson, PD Johann, M Kool, S Pfister, M Hasselblat, MC Frühwald, O Delattre, D Surdez, F Bourdeaut, S Puget, S Zaidi, SS Mitra, S Cheshier, PH Sorensen, M Monje, CL Mackall Locoregionally administered B7-H3-targeted CAR T cells for treatment of atypical teratoid/rhabdoid tumors Nat. Med., 2020-04-27;26(5):712-719. 2020-04-27 [PMID: 32341579] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD276
• Uniprot:
  • NP_079516()