Genetic Strategies: Knockout Validated: B7-2/CD86 Antibody (GL1) [NBP2-30148] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF ...read more
Biological Strategies: Flow Cytometry: B7-2/CD86 Antibody (GL1) [NBP2-30148] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ...read more
Measured by its ability to neutralize B7-2/CD86-induced IL-2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.05-0.25 ug/mL in the presence of 0.5 ug/mL Recombinant Mouse B7-2/CD86 Fc Chimera and 10 ug/mL PHA. (Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. 87:5031).
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CD86/B7-2 as a costimulatory molecule plays a critical role in the activation of naive T-cells and subsequent differentiation by interacting with CD28 and/or CTLA present on T cells. T-cell activation requires both antigen presentation to the T-cell receptor and a second signal mediated by CD80 and CD86 on antigen-presenting cells and CD28 and CTLA-4 on the T cell. CD86 is constitutively expressed on APCs and B cells and under specific conditions on other activated cells. Certain toll like receptor (TLR) ligands, such as LPS (for TLR4) and Poly I:C (for TLR3) have been shown to enhance expression of CD40, CD80, CD86, CD83 and HLA-DR in human papillomavirus (HPV) type 11 E7 loaded monocyte-derived dendritic cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
CD86 - I work in tandem with CD80 CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post.
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