Genetic Strategies: Knockout Validated: B7-2/CD86 Antibody (GL1) [NBP2-30148] - Lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with ...read more
Biological Strategies: Flow Cytometry: B7-2/CD86 Antibody (GL1) [NBP2-30148] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ...read more
Measured by its ability to neutralize B7-2/CD86-induced IL-2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.05-0.25 ug/mL in the presence of 0.5 ug/mL Recombinant Mouse B7-2/CD86 Fc Chimera and 10 ug/mL PHA. (Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. 87:5031).
Read Publication using NBP2-30148 in the following applications:
CD86/B7-2 as a costimulatory molecule plays a critical role in the activation of naive T-cells and subsequent differentiation by interacting with CD28 and/or CTLA present on T cells. T-cell activation requires both antigen presentation to the T-cell receptor and a second signal mediated by CD80 and CD86 on antigen-presenting cells and CD28 and CTLA-4 on the T cell. CD86 is constitutively expressed on APCs and B cells and under specific conditions on other activated cells. Certain toll like receptor (TLR) ligands, such as LPS (for TLR4) and Poly I:C (for TLR3) have been shown to enhance expression of CD40, CD80, CD86, CD83 and HLA-DR in human papillomavirus (HPV) type 11 E7 loaded monocyte-derived dendritic cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
CD86 - I work in tandem with CD80 CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our B7-2/CD86 Antibody (GL1) and receive a gift card or discount.