B3GNT3 Antibody - Azide and BSA Free

Western Blot: B3GNT3 Antibody [NBP2-99178] - Anti-B3GNT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 60 kDa

Immunoprecipitation: B3GNT3 Antibody [NBP2-99178] - B3GNT3 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 ul anti-B3GNT3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-B3GNT3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 64 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

B3GNT3 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human B3GNT3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B3GNT3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for B3GNT3 Antibody - Azide and BSA Free

• B3GALT8
• B3GAL-T8
• B3GNT3
• B3GN-T3
• B3GNT-3
• Beta-1,3-galactosyltransferase 8
• Beta-1,3-GalTase 8
• Beta-1,3-Gn-T3
• Beta-1,3-N-acetylglucosaminyltransferase 3
• beta-1,3-N-acetylglucosaminyltransferase bGnT-3
• beta3GalT8
• Beta3Gal-T8
• beta3Gn-T3
• Beta-3-Gx-T8
• BGnT-3
• Core 1 extending beta-1,3-N-acetylglucosaminyltransferase
• Core1-beta3GlcNAcT
• EC 2.4.1
• EC 2.4.1.-
• HP10328
• putative type II membrane protein
• TMEM3
• TMEM3beta3Gal-T8
• transmembrane protein 3
• UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
• UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Background

B3GNT3 encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B3GNT3