B3GNT3 Antibody

Immunohistochemistry-Paraffin: B3GNT3 Antibody [NBP1-88953] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: B3GNT3 Antibody [NBP1-88953] - Staining of human colon shows high expression.

Immunohistochemistry-Paraffin: B3GNT3 Antibody [NBP1-88953] - Staining in human colon and pancreas tissues using anti-B3GNT3 antibody. Corresponding B3GNT3 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

B3GNT3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B3GNT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: IHC reported in scientific literature (PMID: 24998922). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  B3GNT3 Protein (NBP1-88953PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24998922).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for B3GNT3 Antibody

• B3GALT8
• B3GAL-T8
• B3GNT3
• B3GN-T3
• B3GNT-3
• Beta-1,3-galactosyltransferase 8
• Beta-1,3-GalTase 8
• Beta-1,3-Gn-T3
• Beta-1,3-N-acetylglucosaminyltransferase 3
• beta-1,3-N-acetylglucosaminyltransferase bGnT-3
• beta3GalT8
• Beta3Gal-T8
• beta3Gn-T3
• beta-3-Gx-T8
• BGnT-3
• Core 1 extending beta-1,3-N-acetylglucosaminyltransferase
• core1-beta3GlcNAcT
• EC 2.4.1
• EC 2.4.1.-
• HP10328
• putative type II membrane protein
• TMEM3
• TMEM3beta3Gal-T8
• transmembrane protein 3
• UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
• UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Background

B3GNT3 encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B3GNT3 Antibody (NBP1-88953)(2)

Publications using NBP1-88953

• Li CW, Lim SO, Chung EM, Kim YS. Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1. Cancer Cell. 2018-02-12 [PMID: 29438695] (Human)
• Clark AT, da Costa VM, Costa LB et al. Differential Expression Patterns of N-Acetylglucosaminyl Transferases and Polylactosamines in Uterine Lesions. Eur J Histochem 2014-05-26 [PMID: 24998922] (IHC, Human)

Bioinformatics

• Gene Symbol: B3GNT3