B3GNT3 Antibody

Western Blot: B3GNT3 Antibody [NBP1-32539] - Sample (50 ug of whole cell lysate) A: mouse stomach 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: B3GNT3 Antibody [NBP1-32539] - Paraffin-embedded colon; antibody dilution 1:500.

Western Blot: B3GNT3 Antibody [NBP1-32539] - (30 ug whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

B3GNT3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human B3GNT3. The exact sequence is proprietary.
• Localization: Golgi apparatus membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B3GNT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for B3GNT3 Antibody

• B3GALT8
• B3GAL-T8
• B3GN-T3
• B3GNT-3
• Beta-1,3-galactosyltransferase 8
• Beta-1,3-GalTase 8
• Beta-1,3-Gn-T3
• Beta-1,3-N-acetylglucosaminyltransferase 3
• beta-1,3-N-acetylglucosaminyltransferase bGnT-3
• beta3GalT8
• Beta3Gal-T8
• beta3Gn-T3
• beta-3-Gx-T8
• BGnT-3
• Core 1 extending beta-1,3-N-acetylglucosaminyltransferase
• core1-beta3GlcNAcT
• EC 2.4.1
• EC 2.4.1.-
• HP10328
• putative type II membrane protein
• TMEM3beta3Gal-T8
• transmembrane protein 3
• UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
• UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3

Background

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B3GNT3