B3GALTL Antibody - BSA Free

Western Blot: B3GALTL Antibody [NBP1-32787] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: B3GALTL Antibody [NBP1-32787] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

B3GALTL Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human B3GALTL. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B3GLCT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for B3GALTL Antibody - BSA Free

• B3GALTL
• B3GLCT
• B3Glc-T
• B3GTL
• B3GTLbeta3Glc-T
• beta 1,3-galactosyltransferase-like
• beta 3-glycosyltransferase-like
• beta-1,3-glucosyltransferase
• Beta3Glc-T
• beta-3-glycosyltransferase-like
• EC 2.4.1.-
• Gal-T
• UDP-GAL:beta-GlcNAc beta-1,3-galactosyltransferase-like

Background

B3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1; MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2; MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008 [PubMed 18199743]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: B3GLCT