POFUT2 Antibody - Azide and BSA Free

Western Blot: POFUT2 Antibody [NBP2-93894] - Analysis of extracts of various cell lines, using POFUT2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Immunocytochemistry/Immunofluorescence: POFUT2 Antibody [NBP2-93894] - Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit pAb (NBP2-93894) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: POFUT2 Antibody [NBP2-93894] - Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (NBP2-93894) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

POFUT2 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1). MVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAII
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POFUT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for POFUT2 Antibody - Azide and BSA Free

• C21orf80
• chromosome 21 open reading frame 80
• FUT13EC 2.4.1.221
• GDP-fucose protein O-fucosyltransferase 2
• KIAA0958
• O-FucT-2
• Peptide-O-fucosyltransferase 2
• protein O-fucosyltransferase 2

Background

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: POFUT2