B220/CD45R Antibody (RA3-6B2) [Cy5]

Product Discontinued

B220/CD45R Antibody (RA3-6B2) [Cy5] Summary

• Immunogen: Abelson murine leukemia virus-induced pre-B cell lymphoma
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: PTPRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry, It is recommended that the reagent be titrated for optimal performance for each application. Useful in Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation as reported in Literature.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for B220/CD45R Antibody (RA3-6B2) [Cy5]

• B220
• CD45 antigen
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45R/B220, a member of the protein tyrosine phosphate family and a major cell surface glycoprotein, represents a restricted form of the CD45 family which primarily recognizes only cells of B-lineage from pro-B cell through mature B lymphocytes and, prior to the availability of anti-CD19 MAbs, was commonly used as a pan B-cell marker. (1-5) It also reacts with certain activated T cells, as well as non-MHC-restricted lytically active lymphokine-activated killer (LAK) cells. (1, 6) In vivo administration of RA3-6B2 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice. (7, 8)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC