B-Raf [p Thr401] Antibody (JJ08-72)

Images

 
Western Blot: B-Raf [p Thr401] Antibody (JJ08-72) [NBP2-67846] - Analysis of Phospho-B Raf(T401) on different lysates using anti-Phospho-B Raf(T401) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse testis ...read more
Immunocytochemistry/ Immunofluorescence: B-Raf [p Thr401] Antibody (JJ08-72) [NBP2-67846] - Staining Phospho-B Raf(T401) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunocytochemistry/ Immunofluorescence: B-Raf [p Thr401] Antibody (JJ08-72) [NBP2-67846] - Staining Phospho-B Raf(T401) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunocytochemistry/ Immunofluorescence: B-Raf [p Thr401] Antibody (JJ08-72) [NBP2-67846] - Staining Phospho-B Raf(T401) in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
JJ08-72
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

B-Raf [p Thr401] Antibody (JJ08-72) Summary

Immunogen
Synthetic phospho-peptide surrounding T401 of B Raf
Modification
p Thr401
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRAF
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for B-Raf [p Thr401] Antibody (JJ08-72)

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for B-Raf Antibody (NBP2-67846)

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Blogs on B-Raf.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.

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Bioinformatics

Gene Symbol BRAF