B-Raf Antibody (IHC600) - V600-E Mutation

Immunohistochemistry-Paraffin: B-Raf Antibody (IHC600) - V600-E Mutation [NBP3-00517] - Staining of B-Raf Antibody (IHC600) in thyroid papillary carcinoma with BRAF V600-E mutation

• Reactivity: Hu
• Applications: IHC
• Clone: IHC600
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

B-Raf Antibody (IHC600) - V600-E Mutation Summary

• Description: Novus Biologicals Mouse B-Raf Antibody (IHC600) - V600-E Mutation (NBP3-00517) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A peptide covering the V600E mutation.
• Localization: Cytoplasm
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BRAF
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:30
  • Immunohistochemistry-Paraffin 1:30
• Application Notes: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Colorectal Adenocarcinoma, Thyroid Papillary Carcinoma with the BRAF V600E Mutation.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris Buffer, pH 7.3 - 7.7, 1% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Protein A or G purified from cell culture supernatant

Alternate Names for B-Raf Antibody (IHC600) - V600-E Mutation

• 94 kDa B-raf protein
• B-Raf proto-oncogene serine/threonine-protein kinase (p94)
• BRaf
• B-Raf
• BRAF1
• B-RAF1
• BRAF1MGC126806
• EC 2.7.11.1
• MGC138284
• murine sarcoma viral (v-raf) oncogene homolog B1
• NS7
• p94
• Proto-oncogene B-Raf
• RAFB1
• serine/threonine-protein kinase B-raf
• v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BRAF