Mouse Axl ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Axl (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Axl. |
| Sensitivity |
1 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
AXL |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): 30 ml of animal serum with 0.09% sodium azide as preservative. ForStandard/Sample (serum/plasma) diluent.
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
- Axl Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse Axl.
- Detection Antibody Axl (Item F): 2 vial of biotinylated anti- mouse Axl (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl 4,000x concentrated HRP-conjugated streptavidin.
- Standards (Item C): 2 vials of recombinant mouse Axl.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse Axl ELISA Kit (Colorimetric)
Background
Axl is encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Axl ELISA Kit (KA1776). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.