Human Axl ELISA Kit (Colorimetric) Summary
| Sensitivity |
2 pg/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
AXL |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
AXL (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human AXL. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): Contains 0.09% sodium azide as preservative.
- Assay Diluent B (Item E): 5x concentrated buffer.
- Axl Microplate (Item A): Coated with anti-human Axl
- Detection Antibody Axl (Item F): Biotinylated anti-human Axl (each vial isenough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 300x concentrated HRP-conjugatedStreptavidin.
- Standard Protein (Item C): Human Axl. 1 vial is enough to run each standardin duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
- Wash Buffer Concentrate (20X) (Item B): 20X concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Axl ELISA Kit (Colorimetric)
Background
Axl is encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Axl ELISA Kit (KA1696). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.