Axl Antibody (108724) [Unconjugated]

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Axl knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ELISA(Cap), KO
• Clone: 108724
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Axl Antibody (108724) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Axl
  Met1-Pro440
  Accession # AAA61243
• Specificity: Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AXL

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human Axl Antibody (Catalog # MAB154)
  ELISA Detection: Human Axl Biotinylated Antibody (Catalog # BAF154)
  Standard: Recombinant Human Axl Fc Chimera (Catalog # 154-AL)
• Control:

  Axl Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl Antibody (108724) [Unconjugated]

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (MAB154)(10)

Publications using MAB154

• MJW Peeters, D Dulkeviciu, A Draghi, C Ritter, A Rahbech, SK Skadborg, T Seremet, AM Carnaz Sim, E Martinenai, HR Halldórsdó, MH Andersen, G Holmen Olo, IM Svane, LJ Rasmussen, O Met, JC Becker, M Donia, C Desler, P Thor Strat MERTK acts as a costimulatory receptor on human CD8+ T cells Cancer Immunol Res, 2019;0(0):. 2019 [PMID: 31266785] (Flow Cytometry, Human)
• Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. PLoS Pathog, 2014;10(12):e1004548. 2014 [PMID: 25474532] (Flow Cytometry, Human)
• Micklem D, Blo M, Bergstrom P, Hodneland E, Tiron C, Hoiby T, Gjerdrum C, Hammarsten O, Lorens J Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets. BMC Biotechnol, 2014;14(0):57. 2014 [PMID: 24952598] (Flow Cytometry, Human)
• Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity. Arthritis Res Ther, 0;15(6):R212. 0 [PMID: 24325951] (Human)
• Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. 2012 [PMID: 23071254] (Flow Cytometry, Human)
• Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. J. Virol., 2012;86(4):2067-78. 2012 [PMID: 22156524] (Flow Cytometry, Human)
• Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010;115(23):4963-72. 2010 [PMID: 20348395] (Western Blot, Human)
• Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway. J. Immunol., 2009;183(5):3004-13. 2009 [PMID: 19657094] (Cell Selection, Human)
• Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions. Am. J. Pathol., 2009;175(1):283-93. 2009 [PMID: 19541935] (IHC-Fr, Human)
• Holland SJ, Powell MJ, Franci C, Chan EW, Friera AM, Atchison RE, McLaughlin J, Swift SE, Pali ES, Yam G, Wong S, Lasaga J, Shen MR, Yu S, Xu W, Hitoshi Y, Bogenberger J, Nör JE, Payan DG, Lorens JB Multiple roles for the receptor tyrosine kinase axl in tumor formation. Cancer Res., 2005;65(20):9294-303. 2005 [PMID: 16230391] (Western Blot, Human)

Bioinformatics

• Gene Symbol: AXL
• Uniprot:
  • AAA61243()