Axl Antibody (107332) [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
107332
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Axl Antibody (107332) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Axl
His20-Pro443
Accession # Q00993
Specificity
Detects mouse Axl in ELISAs and Western blots. Does not cross-react with recombinant human (rh) Axl, rmDtk, or rmMer.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
AXL
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Mouse Axl Biotinylated Antibody (Catalog number BAF854)
Standard: Recombinant Mouse Axl Fc Chimera (Catalog number 854-AX)
Reviewed Applications
Read 3 Reviews rated 3.7
using
MAB854 in the following applications:

Publications
Read Publications using
MAB854 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl Antibody (107332) [Unconjugated]

  • AI323647
  • ARK
  • AXL oncogene
  • AXL receptor tyrosine kinase
  • AXL transforming sequence/gene
  • Axl
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK11
  • Tyro7
  • tyrosine-protein kinase receptor UFO
  • UFO
  • UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Axl Antibody (MAB854)(4)

Reviews for Axl Antibody (MAB854) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using MAB854:
Filter by Applications
WB
(1)
Func
(1)
Flow
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot Axl MAB854
Enlarge
reviewed by:
Manjinder Cheema
WB Mouse 02/24/2020
View

Summary

ApplicationWestern Blot
Sample TestedAdult kidney,Liver,Mouse Spleen,Brain
SpeciesMouse
LotHFY0119051

Comments

CommentsNo signal at all in any of he tissues.
Functional Assay Axl MAB854
Enlarge
reviewed by:
Anonymous
Func Mouse 09/25/2018
View

Summary

ApplicationFunctional Assay
Sample Tested Mouse brain
SpeciesMouse
LotHFY0116121
Flow Cytometry Axl MAB854
Enlarge
reviewed by:
Anonymous
Flow Mouse 03/13/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse Cells
SpeciesMouse
LotHFY0116121

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Axl Antibody (MAB854) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Axl Antibody (MAB854)

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Blogs on Axl.

Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.

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Recent Reviews

3.7
5
2
4
0
3
0
2
0
1
1

Manjinder Cheema
02/24/2020
Application: WB
Species: Mouse

Anonymous
09/25/2018
Application: Func
Species: Mouse

Anonymous
03/13/2018
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol AXL
Uniprot
COVID-19 update