Axin-2 Antibody (JM11-30)

Western Blot: Axin-2 Antibody (JM11-30) [NBP2-67175] - Analysis of Axin2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: SW480 cell lysateLane 1: PC-12 tissue lysate Lane 2: NIH/3T3 cell lysate

Immunohistochemistry-Paraffin: Axin-2 Antibody (JM11-30) [NBP2-67175] - Analysis of paraffin-embedded mouse colon tissue using anti-Axin2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Axin-2 Antibody (JM11-30) [NBP2-67175] - Analysis of paraffin-embedded mouse brain tissue using anti-Axin2 antibody. Counter stained with hematoxylin.

Product Discontinued

Axin-2 Antibody (JM11-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Axin-2 aa 55-93 / 843. (SwissProt: Q9Y2T1 Human; SwissProt: O88566 Mouse; SwissProt: O70240 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AXIN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:50
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Axin-2 Antibody (JM11-30)

• AXIL
• axin 2
• Axin2
• Axin-2
• Axin-like protein
• Axis inhibition protein 2
• Conductin
• DKFZp781B0869
• MGC10366
• MGC126582

Background

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AXIN2