Autoimmune Regulator/AIRE Antibody

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.

Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Analysis of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.

• Reactivity: Hu, Po, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Autoimmune Regulator/AIRE Antibody Summary

• Description: Novus Biologicals Goat Autoimmune Regulator/AIRE Antibody (NB100-40799) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Autoimmune Regulator/AIRE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KDVDLSQPRKGRKP corresponding to internal region according to NP_000374.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_000374.1) only.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AIRE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 10 ug/mL
• Application Notes: WB: Approx. 60 kDa band in transfected HEK293 lysates (calculated MW of 57.7 kDa band according to NP_000374.1). IHC-P: Human thymus shows nuclear staining in select thymocytes. IHC usage on fetal porcine thymus is reported in scientific literature (PMID: 24770647).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Autoimmune Regulator/AIRE Antibody

• AIRE
• APECED protein
• APECED
• APECEDAPSI
• APS1
• APSI
• Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein
• autoimmune regulator (APECED protein)10APS1AIRE1
• autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy)
• Autoimmune Regulator
• PGA1

Background

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Autoimmune Regulator/AIRE Antibody (NB100-40799)(1)

Publication using NB100-40799

• Niki S, Oshikawa K, Mouri Y et al. Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice. J Clin Invest 2006-05-01 [PMID: 16628255]

FAQs for Autoimmune Regulator/AIRE Antibody (NB100-40799). (Showing 1 - 1 of 1 FAQ).

1. I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?
   • I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

Bioinformatics

• Gene Symbol: AIRE
• Entrez:
  • 326(Human)
• Uniprot:
  • NP_000374.1(Human)