Recombinant Human Aurora B Protein

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SDS-Page: Aurora B Protein [NBP1-30242] - Aurora Kinase B, 41.4 kDa (364aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Aurora B Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 344 of Human Aurora B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPMGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
AURKB
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
41.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 0.5mM DTT, 20% glycerol, 0.1mM EDTA, 0.1mM EGTA, 0.1M NaCl, 0.1mM PMSF
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Aurora B Protein

  • Aik2
  • AIK2EC 2.7.11.1
  • AIM-1
  • AIM1Aik2
  • AIM-1STK-1
  • ARK2
  • ARK2ARK-2
  • AurB
  • AURKB
  • aurkb-sv2
  • Aurora- and IPL1-like midbody-associated protein 1
  • Aurora B
  • aurora kinase BSerine/threonine-protein kinase aurora-B
  • aurora kinase B-Sv1
  • aurora kinase B-Sv2
  • Aurora/IPL1-related kinase 2
  • aurora-1
  • aurora-B
  • Aurora-related kinase 2
  • EC 2.7.11
  • IPL1
  • serine/threonine kinase 12
  • serine/threonine-protein kinase 12
  • STK12
  • STK12aurkb-sv1
  • STK5

Background

Aurora Kinase B is a member of a family of mitotic serine/threonine kinases. Aurora Kinase B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora Kinase B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of Aurora Kinase B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation, while overexpression in many kinds of cancers. Recombinant human Aurora Kinase B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol AURKB
Entrez