Aurora B Antibody

Product Discontinued

Aurora B Antibody Summary

• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 227-238 of Human Aurora B protein.
• Marker: Mitosis Marker
• Specificity: Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AURKB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 39 kDa in size corresponding - Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control.

Reactivity Notes

Cross reactivity with Aurora B from other species has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.39 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aurora B Antibody

• Aik2
• AIK2EC 2.7.11.1
• AIM1
• AIM-1
• AIM1Aik2
• AIM-1STK-1
• ARK2
• ARK2ARK-2
• AurB
• AURKB
• aurkb-sv2
• Aurora- and IPL1-like midbody-associated protein 1
• Aurora B
• aurora kinase BSerine/threonine-protein kinase aurora-B
• aurora kinase B-Sv1
• aurora kinase B-Sv2
• Aurora/IPL1-related kinase 2
• aurora-1
• aurora-B
• Aurora-related kinase 2
• EC 2.7.11
• IPL1
• serine/threonine kinase 12
• serine/threonine-protein kinase 12
• STK12
• STK12aurkb-sv1
• STK5

Background

Aurora B belongs to Serine/threonine kinase family, Aurora subfamily. Predicted molecular weight 39 kD. This kinase is highly expressed in thymus and many cancers and is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and is up regulated in cancer cells during M phase. Aurora B localizes to centromeres in early mitosis and then the spindle midzone in anaphase and is required for histone H3 phosphorylation, chromosome alignment, centrosome maturation, activation of the spindle assembly checkpoint and cytokinesis. The Poly6361 antibody recognizes human phospho-Aurora B (Thr232) and has been shown to be useful for immunoflorence staining. nbsp

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AURKB
• Uniprot:
  • NP_004208(Human)