ATRX Antibody (CL0537) - Azide and BSA Free

Staining in MCF7 cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in HeLa cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U251 cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in A431 cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining of human glioma shows strong nuclear positivity in tumor cells.

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts and Leydig cells.

Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATRX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0537
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ATRX Antibody (CL0537) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ATRX Antibody (CL0537) - Azide and BSA Free (NBP2-52938) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P46100, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATRX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ATRX Antibody (CL0537) - Azide and BSA Free

• alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog)
• alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae)
• alpha thalassemia/mental retardation syndrome X-linked
• ATP-dependent helicase ATRX
• ATR2
• DNA dependent ATPase and helicase
• EC 3.6.1
• EC 3.6.4.12
• helicase 2, X-linked
• Juberg-Marsidi syndrome
• MGC2094
• MRXHF1
• RAD54 homolog
• RAD54L
• SFM1
• SHS
• transcriptional regulator ATRX
• XH2RAD54
• X-linked helicase II
• X-linked nuclear protein
• XNPZNF-HX
• Zinc finger helicase
• Znf-HX

Background

ATRX is encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATRX